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4In 2007, Barrangou et al. demonstrated for the first time the function of CRISPR/Cas9 as a defensive mechanism of bacteria against bacteriophage attacks by a technique called “plaquing” which builds upon the technique of “plating” bacteria on agar. Plaques of viruses on agar are areas without growth of bacteria due to viral attacks.

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The identification of ‘boosters’ that drive gene overexpression directly in a CAR construct provides a simple and scalable strategy for developing effective CAR-NK cell therapies for solid tumours.

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